TABLE 1:
PHSte5-domain mutants.
| Mutation | Interaction with Ste11 | Mating (% of wild type) |
| R379G | − | — (<<0.1) |
| R407K | Decreased | 9.7 |
| R462E | nd | 92 |
| T465A | Severely decreased | 0.8 |
| K472E | − | — (<<0.1) |
| S484P | Severely decreased | 0.9 |
| Y487H | Decreased | 10.3 |
| N491I | Severely decreased | 0.7 |
| S494P | Severely decreased | 0.7 |
| T498A | + | 100 |
| T499A | nd | 91 |
| Q501R | − | — (<<0.1) |
| K502R | Severely decreased | 0.8 |
| I504T | − | — (<<0.1) |
| L508S | Severely decreased | 0.5% |
| F514L | − | — (<<0.1) |
| WT | + | 100 |
Protein–protein interaction was assayed using the tailed two-hybrid system as described in the text. The relative amount of cell growth reflects the extent of PHSte5-domain interaction with Ste11. nd, not determined.