TABLE 2:
RBLSte11-domain mutants.
| Mutation | Mating (% of wild type) | HOG pathway |
|---|---|---|
| G128D | — (<<0.1) | + |
| V176R | 0.4 | + |
| D178R | 91 | + |
| V193A | — (<<0.1) | + |
| N199S | — (<<0.1) | + |
| E203G | — (<<0.1) | + |
| N126T, K225I | — (<<0.1) | + |
| T166A, D189G | — (<<0.1) | + |
| D173R, F175A | 97 | + |
| Y188C, S241R | 0.7 | + |
| WT | 100 | + |
Semiquantitative mating assay was performed with yeast cells (ste11Δ) carrying the Ste11 alleles indicated. Mating efficiency is expressed as the percentage of the wild type. The HOG pathway activity was assayed on hyperosmotic medium as described (Wu et al. 1999) for growth of yeast cells (ste11Δ ssk2Δ ssk22Δ) carrying the Ste11 alleles.