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. 2013 Feb 1;24(3):184–193. doi: 10.1091/mbc.E12-09-0651

FIGURE 2:

FIGURE 2:

Stable knockdown of C7orf30 results in a mitochondrial translation defect. Pulse-chase labeling of newly synthesized mitochondrial polypeptides (at right; ND, subunits of complex I; CO, subunits of complex IV; cyt b, subunit of complex III; ATP, subunits of complex V) in control and C7orf30-shRNA-A2-C2 fibroblasts alone or expressing C7orf30-FLAG. The number in parentheses is the ratio of individual band intensities of the shRNA-A2-C2 lane over the shRNA-A1 (control) lane. SDHA is used as a loading control on an SDS–PAGE immunoblot of the same samples.