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. 2013 Feb 1;24(3):297–307. doi: 10.1091/mbc.E12-02-0081

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© 2013 Zheng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Notch encodes an endosomal sorting signal. (A) Diagram illustrating Notch domain structure of the cytoplasmic tail and CD8-NΔE truncation mutants used for analysis. (B) Immunoblot analysis indicating expression of each CD8-NΔE form in tTA HeLa cells. (C) Immunolocalization analysis of the Notch cytoplasmic tail following infection with adenovirus encoding the indicated CD8-NΔE form (CD-NΔE, CD8-NΔ2194, CD8-NΔ2080). (D) Quantitation of cells forming enlarged endosomes (EE) following overexpression of the indicated Notch form. Cells were infected with adenovirus encoding the indicated CD8-NΔE control or truncation mutant. For each condition, more than 200 cells were counted. Boxed region indicates area of higher magnification. Error bars represent ± SEM. Scale bar: 10 μm.