FIGURE 3:

A conserved dileucine motif directs Notch endosomal sorting. (A) Amino acid alignment of the Notch cytoplasmic tail spanning the dileucine motif. Underlined region indicates the conserved dileucine sorting motif that follows the [D/E]XXX[L][L/I] pattern. tTA HeLa cells were infected with adenovirus encoding either CD8-NΔE (B, D, F, and H) or CD8-NΔE LLAA (C, E, G, and I) and processed for immunolocalization. Permeabilized cells (B–G) were probed with antibodies to the NICD, while nonpermeabilized cells (H and I) were probed with the mAb 51.1 to detect the CD8 epitope at the cell surface. Boxed regions indicate areas of higher magnification. Scale bar: 20 μm (B,C); 10 μm (D–I).