FIGURE 4:

Notch internalization is independent of the dileucine motif. (A) Notch cell-surface levels were measured by flow cytometry (see Materials and Methods) in CD8-NΔE– or CD8-NΔE LLAA–overexpressing tTA HeLa cells, which were pretreated with control siRNA or siRNA against clathrin heavy chain (CHC). (B) The corresponding signaling for each Notch chimera was measured by luciferase assay in the presence or absence of 1 μM CE. Error bars represent ± SEM; p values by t test: *, p < 0.05; **, p < 0.005.