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. 2013 Feb 1;24(3):297–307. doi: 10.1091/mbc.E12-02-0081

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© 2013 Zheng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — CD8-NΔE LLAA accumulates in CD-MPR–positive tubular endosomes. PEN1/2^−/− MEFs were cotransfected with plasmid encoding SARA-mChr (A and D) and CD8-NΔE-GFP (B) or CD8-NΔE LLAA-GFP (E). Alternatively, cells were cotransfected with CD-MPR-GFP (G and J) and CD8-NΔE-mChr (H) or CD8-NΔE LLAA-mChr (K). Live cells were imaged after a 10-h incubation to limit recombinant protein expression levels. Merged images are shown in (C), (F), (I), and (L). Scale bar: 10 μm.