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. Author manuscript; available in PMC: 2013 Feb 5.
Published in final edited form as: Transfusion. 2011 Oct 27;52(5):1112–1116. doi: 10.1111/j.1537-2995.2011.03411.x

Fig. 2.

Fig. 2

Reactions of HNA-3a and HNA-3b antibodies with HEK293 cells expressing recombinant CTL2. Studies were done using flow cytometry and 10 μL of serum containing HNA-3a antibodies from 20 blood donors implicated in TRALI reactions (designated 1–20) and one patient with an HNA-3b antibody (designated 3b). HNA-3a antibodies were tested in triplicate and the HNA-3b antibody was tested in duplicate. Each of the 20 HNA-3a antibodies reacted preferentially with cells expressing CTL2 (R154) (dark bars) and the single example of anti-HNA-3b reacted preferentially with CTL2 (Q154) (light bars). N’Ave = reactions of four unselected normal sera. Results shown are the median ± 1.0 SD. Significance of the differences observed (R154 vs. Q154) is indicated as *p < 0.05, **p < 0.01, and ***p < 0.001.