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. 2012 Feb 15;32(7):2398–2409. doi: 10.1523/JNEUROSCI.6056-11.2012

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Copyright © 2012 the authors 0270-6474/12/322398-12$15.00/0

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Figure 5. — Striatal expression of the dominant-negative Kir2.1^AAA channel is sufficient to induce MSN hyperexcitability and dendritic atrophy. a, Representative coronal section showing GFP autofluorescence 1 month after viral injection in the dorsomedial striatum (2.5× magnification). Insets show AAV2/1-GFP infected MSNs from a slice recording and coordinates of injections. b, Inward currents at negative voltages obtained for GFP- and Kir2.1^AAA-infected control and D2R-OE MSNs. There is a significant effect of genotype (p < 0.0001) and of Kir2.1^AAA expression (p < 0.0001). c, Distribution of basic electrical properties of GFP- and Kir2.1^AAA-expressing MSNs in control and D2R-OE mice (bars, min/max values; box, lower/upper quartile; line, median). ***p < 0.001, individual groups compared with control + GFP; ^###p < 0.001, D2R-OE + Kir2.1^AAA compared with D2R-OE + GFP. d, e, Current–voltage (d) and input–output (e) curves are significantly altered (overall group effect: d, p < 0.0001; e, p < 0.0001). Individual comparisons show significant changes in the three test groups (control + Kir2.1^AAA, D2R-OE + GFP, D2R-OE + Kir2.1^AAA), compared with the control + GFP group (all p < 0.05). There was no significant difference between the test groups. Control + GFP, n = 26 cells (5 mice); D2R-OE + GFP, n = 36 (5); control + Kir2.1^AAA, n = 29 (6); D2R-OE + Kir2.1^AAA, n = 30 (6). f, Representative biocytin-filled MSNs and corresponding Neurolucida reconstructions from control mice injected with either GFP or Kir2.1^AAA virus (40× magnification). Gray circle radius, 50 μm. g, h, 3-D Sholl analysis demonstrates a change in cumulative length (g) and complexity (h) (overall group effect g, p < 0.005; h, p < 0.0005). Individual comparisons show significant decreases in the three test groups (control + Kir2.1^AAA, D2R-OE + GFP, D2R-OE + Kir2.1^AAA) compared with the control + GFP group (all p < 0.05). There were no significant differences between the test groups. Control + GFP, n = 21 (5); D2R-OE + GFP, n = 30 (5); control + Kir2.1^AAA, n = 21 (6); D2R-OE + Kir2.1^AAA, n = 22 (6).