Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jan 30;69(Pt 2):122–125. doi: 10.1107/S1744309112050300

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© International Union of Crystallography 2013

PMC Copyright notice

Purification of the refolded BF2*1501 complex (BF2*1501, chicken β₂m and the epitope MDV-MEQ_RRR9) by FPLC Superdex 200 16/60 HiLoad gel-filtration and Resouce-Q anion-exchange chromatography (GE Healthcare). (a) Gel-filtration profile of the refolded products. Peak 1 corresponds to the aggregated BF2*1501 heavy chain, peak 2 corresponds to the correctly refolded complex (∼44 kDa) and peak 3 corresponds to excess chicken β₂m. Inset, reduced SDS–PAGE gel (15%) for peaks 1, 2 and 3. Lane M contains molecular-mass markers (labelled in kDa). (b) Anion-exchange chromatography profile of the refolded products. Peak 1 corresponds to correctly refolded complex, which was eluted at an NaCl concentration of 16–20%. Inset, reduced SDS–PAGE gel (15%) for peak 1. Lane M contains molecular-mass markers (labelled in kDa).