Table 1. The 11 test proteins with their formulation buffers and concentrations.
EDTA, ethylenediaminetetraacetic acid; PMSF, phenylmethanesulfonyl fluoride.
| Protein | Concentration (mgml1) | Buffer | No. of tryptophans | No. of residues | Comments |
|---|---|---|---|---|---|
| Catalase | 30 | 25mM HEPES pH 7 | 6 | 506 | Haem protein |
| Haemoglobin | 40 | 100mM NaH2PO4 pH 6.5 | 1 | 141 | Haem protein |
| Myoglobin | 20 | H2O | 2 | 153 | Haem protein |
| Ferritin | 20 | 50mM NaCl | 1 | 183 | Iron-containing |
| Ribonuclease A | 30 | 100mM sodium acetate pH 6 | 0 | 124 | Tryptophan-free |
| Insulin | 20 | 20mM Na2HPO4, 10mM Na3EDTA | 0 | 21 | Tryptophan-free |
| Proteinase K | 20 | 25mM Tris pH 7.5, 1mM PMSF | 2 | 279 | |
| Thaumatin | 50 | 100mM sodium/potassium tartrate | 3 | 207 | |
| Thermolysin | 25 | 50mM NaOH | 3 | 316 | |
| Apoferritin | 20 | H2O | 1 | 174 | |
| Concanavalin A | 50 | H2O | 4 | 237 |