Skip to main content
. 2013 Jan 26;69(Pt 2):201–208. doi: 10.1107/S1744309112048634

Table 2. The test proteins and solutions used for the UVVis spectra with their buffers and concentrations.

DMSO, dimethyl sulfoxide.

Protein or solution Concentration (mgml1) Buffer
Catalase 7.5 25mM HEPES pH 7
Haemoglobin 1.25 100mM Na/H2PO4 pH 6.5
Myoglobin 1.25 H2O
Ferritin 0.625 50mM NaCl
Ribonuclease A 15 100mM sodim acetate pH 6
Insulin 10 20 mM Na2/HPO4 10mM Na3EDTA
Proteinase K 10 25mM Tris pH 7.5, 1mM PMSF
Thaumatin 6.25 100mM sodium/potassium tartrate
Thermolysin 3.125 50mM NaOH
Apoferritin 5 H2O
Concanavalin A 6.25 H2O
Haematin 0.158 DMSO
FeCl3 1.6 H2O
Sodium nitrate 60 H2O