Table 3. The four proteins used in the 768-condition screen.

TBS, Tris-buffered saline. TCEP, tris(2-carboxyethyl)phosphine. The four proteins used inthis study were samples from clients of C3 and were not part of the test set of commercially available proteins.

Protein	No. of tryptophans	Concentration (mgml1)	Buffer	Size (kDa)
A	4	10	TBS pH 8	20.3
B	3	10	TBS pH 8	20.5
C	6	10	TBS pH 8	42.7
D	2	5.3	200mM NaCl, 20mM Tris pH 8, 10% glycerol, 0.5mM TCEP	32.9