Table 1. Composition of the 48 buffer screen solutions.
The concentrations given are the final concentrations used during the experiment.
| Well | Buffer | Salt |
|---|---|---|
| A1 | Water | |
| A2 | 50 mM sodium citrate pH 4.0 | |
| A3 | 50 mM sodium acetate pH 4.5 | |
| A4 | 50 mM sodium citrate pH 5.0 | |
| A5 | 50 mM sodium citrate pH 5.5 | |
| A6 | 50 mM bis-tris pH 6.0 | |
| A7 | 50 mM sodium phosphate pH 6.0 | |
| A8 | 50 mM MES pH 6.2 | |
| A9 | 50 mM bis-tris propane pH 6.5 | |
| A10 | 50 mM ADA pH 6.5 | |
| A11 | 50 mM MES pH 6.7 | |
| A12 | 50 mM PIPES pH 6.7 | |
| B1 | 50 mM sodium phosphate pH 7.0 | |
| B2 | 50 mM MOPS pH 7.0 | |
| B3 | 50 mM HEPES pH 7.0 | |
| B4 | 50 mM HEPES pH 7.5 | |
| B5 | 50 mM Tris–HCl pH 7.5 | |
| B6 | 50 mM HEPES pH 8.0 | |
| B7 | 50 mM Tris–HCl pH 8.0 | |
| B8 | 50 mM Bicine pH 8.0 | |
| B9 | 50 mM Tris–HCl pH 8.5 | |
| B10 | 50 mM CHES pH 9.0 | |
| B11 | 50 mM CHES pH 9.5 | |
| B12 | 50 mM CHES pH 10.0 | |
| C1 | 250 mM NaCl | |
| C2 | 50 mM sodium citrate pH 4.0 | 250 mM NaCl |
| C3 | 50 mM sodium acetate pH 4.5 | 250 mM NaCl |
| C4 | 50 mM sodium citrate pH 5.0 | 250 mM NaCl |
| C5 | 50 mM sodium citrate pH 5.5 | 250 mM NaCl |
| C6 | 50 mM bis-tris pH 6.0 | 250 mM NaCl |
| C7 | 50 mM sodium phosphate pH 6.0 | 250 mM NaCl |
| C8 | 50 mM MES pH 6.2 | 250 mM NaCl |
| C9 | 50 mM bis-tris propane pH 6.5 | 250 mM NaCl |
| C10 | 50 mM ADA pH 6.5 | 250 mM NaCl |
| C11 | 50 mM MES pH 6.7 | 250 mM NaCl |
| C12 | 50 mM PIPES pH 6.7 | 250 mM NaCl |
| D1 | 50 mM sodium phosphate pH 7.0 | 250 mM NaCl |
| D2 | 50 mM MOPS pH 7.0 | 250 mM NaCl |
| D3 | 50 mM HEPES pH 7.0 | 250 mM NaCl |
| D4 | 50 mM HEPES pH 7.5 | 250 mM NaCl |
| D5 | 50 mM Tris–HCl pH 7.5 | 250 mM NaCl |
| D6 | 50 mM HEPES pH 8.0 | 250 mM NaCl |
| D7 | 50 mM Tris–HCl pH 8.0 | 250 mM NaCl |
| D8 | 50 mM Bicine pH 8.0 | 250 mM NaCl |
| D9 | 50 mM Tris–HCl pH 8.5 | 250 mM NaCl |
| D10 | 50 mM CHES pH 9.0 | 250 mM NaCl |
| D11 | 50 mM CHES pH 9.5 | 250 mM NaCl |
| D12 | 50 mM CHES pH 10.0 | 250 mM NaCl |