Figure 4.

Quantitative gene expression of B-class genes in dissected floral organs. Expression of both PISTILLATA (PI) homologs is constrained to whorls 2 and 3 of Clermontia arborescens (top two right panels) but is extended to whorls 1, 2 and 3 in C. parviflora (top two left panels). No substantial difference in expression pattern is observed in other B-function genes, AP3 and TM6 homologs (bottom two panels). β-actin was used as an internal control. Bars indicate mean and standard deviation for three independent experiments each including two replicates. 1, outer perianth; 2, inner perianth; 3, stamen; 4, carpel; L, leaf; H, hypanthium.