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. 2013 Jan 7;13:3. doi: 10.1186/1471-2180-13-3

Table 1.

Spacers characteristics used in this study

Name Genome position* Framing genes* PCR primers PCR product size (bp)
Spacer 1
 106145-106396
 MAB_0104:enoyl-CoA hydratase/isomerise
 F : GGGATGCGCAGATGACGGGG
 506
MAB_0105c:oxidoreductase
 R : GCTACCCCGAATGGGGCACG
Spacer 2
 173727-173985
 MAB_0176:antigen 85-A precursor
 F : TCGAGTTTCCTCCGGGCGGT
 438
MAB_0177:antigen 85-A/B/C precursor
 R: AATCCAGGCAGAACGGCCGC
Spacer 3
 422777-423027
 MAB_0423c:hypothetical protein
 F: GCCATTGCTGTCCGTGCGGT
 344
MAB_0424:putative protease
 R : GCCGCGAACAGGCCAAACAG
Spacer 4
 494411-494670
 MAB_0495c:hypothetical protein
 F: CGCCCTTGCGCAGGAGTGAT
 528
MAB_0496c:hypothetical protein
 R: GCCTGGTTCGGACGGTGACG
Spacer 5
 761805-762060
 MAB_0761c:putative 3-hydroxyacyl-CoA dehydrogenase
 F : ACCACATCGGCGAGCGTGTG
 545
MAB_0762:hypothetical protein
 R : CCAACACCGGGTCGCGGTAC
Spacer 6
 771170-771436
 MAB_0772c:hypothetical protein
 F : CGTCGGTCTTGCCGACCGTC
 600
MAB_0773:hypothetical protein
 R : GGCGCCGACGATCTAGCACC
Spacer 7
 880381-880639
 MAB_0887c:hypothetical protein
 F: CGGCAGTGCAAGGTGCGTTG
 519
MAB_0888c:putative fumarylacetoacetase
 R : GCACCGTGTCCGGTCCTCAG
Spacer 8
 959422-959678
 MAB_0950c:putative amino acid permease family protein
 F: GGGGCGTATGCGCCGTTACC
 474
MAB_0951:putative aminoglycoside phosphotransferase
 R : CGAACGCGCTGTGATTCGGC
Spacer 9
 1002935-1003200
 MAB_0995:hypothetical protein
 F : GGCCGCGACAAGCTGATCGT
 684
MAB_0997c:hypothetical protein
 R: ATGCAGGGCACCGTGCGTAG
Spacer 10
 1216613-1216879
 MAB_1201c:transcription elongation factor GreA
 F: CGTTCTCGCGCAGGTCTCCC
 517
MAB_1202c:hypothetical protein
 R: CCGAACGATCCGTGCCGGTC
Spacer 11 1818877-1819188 MAB_1818:hypothetical protein
 F: AGCCAACTGCCATGGCGCTT
 495
MAB_1819c:hypothetical protein R : ACCGAGACGTCATGCACCGC

* With reference to M. abscessus ATCC 19977 genome.