Table 1.

Targeted genes and list of primers used in this study

Pathogen	Gene	Primer sequence (5′-3′)	Size of PCR product (bp)	Reference	Positive controls
Borrelia burgdorferi s.l.	OspA	GGG AAT AGG TCT AAT ATT AGC CCAC TAA TTG TTA AAG TGG AAG T	665 bp	[26]	ATCC BG/V
Anaplasma phagocytophilum	msp2	TTG GTC TTG AAG CGC TCG TAATG GAA GGT AGT GTT GGT TAT GGT ATT	420 bp	[27]	A. phagocytophilum (dog isolate)
Ehrlichia canis	16S rRNA	Ehrlichia spp.AGA ACG AAC GCT GGC GGC AAG CCCGT ATT ACC GCG GCT GCT GGC	478 bp	[29]	E. canis (dog isolate)
		Ehrlichia canis CAA TTA TTT ATA GCC TCT GGC TAT AGCTAT AGG TAC CGT CAT TAT CTT CCC TAT	389 bp
Coxiella burnetii	IS1111	CTC GTA ATC ACC AAT CGC TTC GCAA GAA TGA TCG TAA CGA TGC GC	337 bp	[28]	105C. burnetii/ml (culture)