Table 1.
A 30-plex gene expression panel, including five human gene normalization controls and the two human ribosomal (DNA and RNA) controls used in singleplex
| Gene | Gene name | GenBank accession | Rationale |
|---|---|---|---|
| 18S ribosomal DNA |
. |
NR_003286 |
Tissue Quality & Quantity Assessment |
| 28S ribosomal RNA |
. |
NR_003287 |
“ “ |
|
ACTB |
Actin, beta |
NM_001101 |
Normalization |
|
PPIA |
Peptidylprolyl isomerase A (cyclophilin A) |
NM_021130 |
“ “ |
|
RPL13A |
Ribosomal protein L13a |
NM_012423 |
“ “ |
|
TBP |
TATA box binding protein |
NM_003194 |
“ “ |
|
UBC |
Ubiquitin C |
NM_021009 |
“ “ |
|
ACTA2 |
Actin, alpha 2, smooth muscle, aorta |
NM_001613 |
Fibrosis and/or HSC Activation Marker |
|
ANXA2 |
Annexin A2 |
NM_001002857 |
“ “ |
|
ARNT2 |
Aryl-hydrocarbon receptor nuclear translocator 2 |
NM_014862 |
HSC Housekeeping |
|
COL1A1 |
Collagen, type I, alpha 1 |
NM_000088 |
Fibrosis and/or HSC Activation Marker |
|
COL3A1 |
Collagen, type III, alpha 1 |
NM_000090 |
“ “ |
|
COL4A1 |
Collagen, type IV, alpha 1 |
NM_001845 |
“ “ |
|
CPEB1 |
Cytoplasmic polyadenylation element binding protein 1 |
NM_030594 |
HSC Housekeeping |
|
CTGF |
Connective tissue growth factor |
NM_001901 |
Fibrosis and/or HSC Activation Marker |
|
CTHRC1 |
Collagen triple helix repeat containing 1 |
NM_138455 |
“ “ |
|
CXCL2 |
Chemokine (C-X-C motif) ligand 2 ; CINC-2a, GROb, Gro2, MIP-2, MIP-2a |
NM_002089 |
“ “ |
|
EDN1 |
Endothelin 1 |
NM_001955 |
“ “ |
|
FOXF1 |
Forkhead box F1 |
NM_001451 |
HSC Housekeeping |
|
HAMP |
Hepcidin antimicrobial peptide |
NM_021175 |
Fibrosis and/or HSC Activation Marker |
|
HSPA1A |
Heat shock 70 kDa protein 1A |
NM_005345 |
“ “ |
|
IGFBP6 |
Insulin-like growth factor binding protein 6 |
NM_002178 |
HSC Housekeeping |
|
KRT18 |
Keratin 18; cytokeratin 18, cell proliferation inducing protein 46 |
NM_000224 |
Fibrosis and/or HSC Activation Marker |
|
LOXL2 |
Lysyl oxidase-like 2 |
NM_002318 |
“ “ |
|
MRGPRF |
MAS-related GPR, member F |
NM_145015 |
HSC Housekeeping |
|
P311 |
Neuronal protein 3.1, C5orf13 |
NM_004772 |
“ “ |
|
SYP |
Synaptophysin |
NM_003179 |
Fibrosis and/or HSC Activation Marker |
|
TGFB1 |
Transforming growth factor, beta 1 |
NM_000660 |
“ “ |
|
TGFB2 |
Transforming growth factor, beta 2 |
NM_003238 |
“ “ |
|
TIMP1 |
TIMP metallopeptidase inhibitor 1 |
NM_003254 |
“ “ |
|
TNF |
Tumor necrosis factor (TNF superfamily, member 2) |
NM_000594 |
“ “ |
| TUBB | Tubulin, beta | NM_178014 | HSC Housekeeping & Fibrosis Marker |