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. 2013 Feb 5;8(2):e55485. doi: 10.1371/journal.pone.0055485

Figure 5. Synergism of cellulase in the presence of the CelA enzyme.

Figure 5

The reaction mixture (200 µl final volume) contained 0–1 mg Accellerase 1500 protein per g of Avicel, 1.0% (w/v) Avicel, and 0–10 mg CelA protein per g of Avicel in 0.05M acetate buffer (pH 5.0). The mixture was shaken at 50°C for 1 h before quantification of reducing sugars; sample 1, cell lysate of the E. coli BL21 cells harboring plasmid pET28, sample 2, cell lysate of the E. coli BL21 cells producing recombinant FX003685 enzyme.