Table 4.

Correlation of CvP-bias differences with experimentally determined Tm value differences for various orthologous protein pairs

Protein		Thermophilic (A. pompejana or C. thermophilum)	Mesophilic (human,S. cerevisiaeorT. reesei)	Difference
Rrp4 (A. pompejana vs. human)	CvP-bias	0.408	6.122	5.714
	Tm	38°C	43.1°C	5.1
Pol η (A. pompejana vs. human)	CvP-bias	5.71	3.67	−2.04
	Tm	49°C	43°C	−6
Collagen (A. pompejana vs. human)	CvP-bias	8.696	5.804	−2.892
	Tm	45-46°C	38-42°C	−3 to −8
U2AF65 (A. pompejana vs. human)	CvP-bias	11.90	5.68	−6.22
	Tm	47°C	43°C	−4
Nup170 (C. thermophilum vs. S. cerevisiae)	CvP-bias	−1.13	−4.86	−3.73
	Tm	57°C	36°C	−21
Nup192 (C. thermophilum vs. S. cerevisiae)	CvP-bias	3.47	−3.33	−6.8
	Tm	57°C	36°C	−21
Xylanase (C. thermophilum vs. Trichoderma reesei)	CvP-bias	−20.276	−22.523	−2.24
	Tm	60°C	50°C	−10

Validation of CvP-bias with experimentally determined thermal stabilities for orthologous protein pairs from refs. [8,30].