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GFP-TM1 does not interact with coexpressed viral Env. Immature HIV-1 bearing both GFP-TM1 and JRFL ΔCT Env was treated with or without 0.5% TX100. Anti-CT, anti-gp120 and anti-CA antibodies were used to detect GFP-TM1, ΔCT Env (unprocessed gp140 or processed gp120), and Gag (MA/p6 - full length, MA/CA – full length missing p6), respectively.
