Table 2.
Similarities between yeast and human SCD proteins
|
Selection of yeast SCD genes with human ortholog SCD genes | ||
|---|---|---|
| Yeast | Human | Function |
|
Dna2* |
(DNA2) |
Nuclease and helicase required for Okazaki fragment processing; involved in DNA repair. |
|
Fkh2 |
(FOXJ3) |
Transcription factor with a major role in the expression of G2/M phase genes. |
|
Gcn2* |
(EIF2AK4) |
Protein kinase, phosphorylates eIF2 (Sui2p) in response to starvation; contributes to DNA damage checkpoint control. |
|
Pol5 |
(MYBBP1A) |
DNA Polymerase phi; not required for chromosomal DNA replication; required for the synthesis of rRNA. |
|
Nup100/116 |
(POM121) |
Subunit of the nuclear pore complex (NPC); interacts with mRNA export factor Mex67p and with Kap95p. |
|
Rad54* |
(RAD54L) |
Involved in the recombinational repair of double-strand breaks. |
|
Rfx1 |
(RFX1) |
Major transcriptional repressor of DNA-damage-regulated genes. |
|
Smc2 |
(SMC2) |
Subunit of the condensin complex. |
|
Taf9** |
(TAF9B) |
Subunit of TFIID and SAGA complexes. |
|
Tif4631* |
(EIF4G2) |
Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F). |
|
Selection of yeast SCD genes whose human ortholog contains phosphorylated S/T-Q sites | ||
|
Bub1 |
(BUB1) |
Protein kinase that play crucial roles in the Spindle Assembly Checkpoint. |
|
Cdc20 |
(CDC20) |
Cell-cycle regulated activator of APC/C, which is required for metaphase/anaphase transition. |
|
Cdc4 |
(FBXW7) |
F-box protein that controls cell cycle function, sulfur metabolism, and methionine biosynthesis. |
|
Hrr25 |
(CSNK1D) |
Protein kinase involved in regulating vesicular trafficking, DNA repair, and chromosome segregation. |
|
Mad1 |
(MAD1) |
Coiled-coil protein involved in the spindle-assembly checkpoint. |
|
Mcm1 |
(SRF) |
Transcription factor involved in cell-type-specific transcription and pheromone response. |
|
Mcm6 |
(MCM6) |
Protein involved in DNA replication; component of the Mcm2-7 hexameric pre-replicative complex. |
|
Mih1* |
(CDC25A) |
Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p. |
|
Mlh1** |
(MLH1) |
Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis. |
|
Msh3 |
(MSH3) |
Mismatch repair protein. |
|
Pds5** |
(APRIN) |
Protein required for establishment and maintenance of sister chromatid condensation and cohesion. |
|
Sch9 |
(RPS6KA1) |
Kinase involved in transcriptional activation of osmostress-responsive genes; regulates G1 progression. |
|
Tid3** |
(NDC80) |
Part of the kinetochore-associated Ndc80 complex involved in chromosome segregation. |
|
Ubr1 |
(UBR1) |
E3 ubiquitin ligase (N-recognin) that ubiquitinate substrates in the N-end rule pathway. |
|
Ycs4** |
(NCAPD2) |
Subunit of the condensin complex. |
|
Selection of yeast SCD genes whose human ortholog contains an SCD and is known to be phosphorylated in S/T-Q sites | ||
|
Akl1* |
(AAK1) |
Protein kinase involved in endocytosis and actin cytoskeleton organization. |
|
Bni1 |
(FMN2) |
Formin, nucleates the formation of linear actin filaments; involved in and mitotic spindle orientation. |
|
Cdc3** |
(SEP6*) |
Component of the septin ring of the mother-bud neck that is required for cytokinesis. |
|
Ctf18** |
(CHTF18) |
Required for sister chromatid cohesion; part of the DNA damage replication checkpoint. |
|
Exo1 |
(EXO1*) |
5′-3′ exonuclease and flap-endonuclease involved in recombination, DSB and mismatch repair. |
|
Pol2 |
(POLE) |
Catalytic subunit of DNA polymerase (II) epsilon. |
|
Rad50* |
(RAD50*) |
Subunit of MRX complex involved in processing double-strand DNA breaks, and telomere maintenance. |
|
Rts1 |
(PPP2RA*) |
Regulatory subunit of protein phosphatase 2A (PP2A). |
| Tom1** | (HUWE1*) | E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus. |
* Phosphorylated in S/T-Q sites in mass spectrometry searches.
** Phosphorylated in S/T-Q sites within an SCD in mass spectrometry searches.