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. 2013 Feb 5;8(2):e55378. doi: 10.1371/journal.pone.0055378

Figure 5. PDK1 is essential for B cell activation.

Figure 5

(A) Phosphorylation of AKT at T308 and S473 in primary B cells stimulated with AffiniPure F(ab′)2 fragment goat anti-Mouse IgM and with or without 3-HAA were analyzed by immunoblot anlaysis. (B) Phosphorylation of JNK and p38 MAPK and IκBα degradation in primary B cells stimulated with anti-IgM antibody and with or without 3-HAA were analyzed by immunobloting analysis. (C) Flow cytometric analyses of B cell activation markers (CD69 and CD86) were performed with primary B cells stimulated with anti-IgM antibody for 24 hours and with or without 3-HAA. Numbers indicate the percentage of CD69 or CD86 positive cells. DMSO was used for the vehicle control. (A), (B) and (C) are representative of three independent experiments.