Figure 4. Most DNA present in the MEE was negative for actin, indicating it was actively produced and not released due to necrosis.

MEE were stained with phalloidin to detect actin presence. MEEs were treated with the nucleic acid stain SYTO9 (green) and TRITC labelled Phalloidin (red). A) No red fluorescence was observed in most samples indicating actin was not present. This compared to B) where some actin is present (red). Even when the samples were positive for actin, this was not extensive, suggesting a combination of active DNA release and the presence of necrotic neutrophils is likely.