Table 2. Comparison of peptides’ EIC peak areas of Lys-C digested non-reduced MAb-A Lot B and Lot B Peak 1 Fraction.
Peptidea | Normalizedb peak area |
||
---|---|---|---|
MAb-A Lot B | Lot B Peak 1 |
Peak 1/ MAb-A |
|
H25 |
1.00 |
1.00 |
1.0 |
H6 |
1.61 |
1.60 |
1.0 |
H26 |
1.83 |
1.81 |
1.0 |
L5 |
1.70 |
2.40 |
1.4 |
L8 |
0.90 |
1.20 |
1.3 |
L9 |
1.22 |
1.80 |
1.5 |
H7-S-S-H8 |
1.28 |
1.31 |
1.0 |
H13-S-S-H13 (inter-chain) |
0.15 |
0.14 |
0.9 |
H14-S-S-H18 |
1.01 |
0.78 |
1.0 c |
H14(ox.M252)-S-S-H18 |
0.02 |
0.22 |
|
H24-S-S-H28 |
0.50 |
0.41 |
|
H24-S-S-H28(ox.M428) |
0.01 |
0.11 |
1.0 c |
L13-S-S-H12 (inter-chain) |
0.58 |
0.57 |
1.0 |
L6-S-S-L12 |
0.91 |
1.01 |
|
L6(clipped)-S-S-L12 | 0.00 | 0.33 | 1.5c |
a H, heavy chain, L, light chain, -S-S-, disulfide linked peptides. bNormalized by dividing the peak area of peptide H25 in the same sample. cThe ratio of sums of expected and oxidized or clipped peptides.