Table 2. Comparison of peptides’ EIC peak areas of Lys-C digested non-reduced MAb-A Lot B and Lot B Peak 1 Fraction.
| Peptidea | Normalizedb peak area |
||
|---|---|---|---|
| MAb-A Lot B | Lot B Peak 1 |
Peak 1/ MAb-A |
|
| H25 |
1.00 |
1.00 |
1.0 |
| H6 |
1.61 |
1.60 |
1.0 |
| H26 |
1.83 |
1.81 |
1.0 |
| L5 |
1.70 |
2.40 |
1.4 |
| L8 |
0.90 |
1.20 |
1.3 |
| L9 |
1.22 |
1.80 |
1.5 |
| H7-S-S-H8 |
1.28 |
1.31 |
1.0 |
| H13-S-S-H13 (inter-chain) |
0.15 |
0.14 |
0.9 |
| H14-S-S-H18 |
1.01 |
0.78 |
1.0 c |
| H14(ox.M252)-S-S-H18 |
0.02 |
0.22 |
|
| H24-S-S-H28 |
0.50 |
0.41 |
|
| H24-S-S-H28(ox.M428) |
0.01 |
0.11 |
1.0 c |
| L13-S-S-H12 (inter-chain) |
0.58 |
0.57 |
1.0 |
| L6-S-S-L12 |
0.91 |
1.01 |
|
| L6(clipped)-S-S-L12 | 0.00 | 0.33 | 1.5c |
a H, heavy chain, L, light chain, -S-S-, disulfide linked peptides. bNormalized by dividing the peak area of peptide H25 in the same sample. cThe ratio of sums of expected and oxidized or clipped peptides.