Table 3. C^1/2_SUPREX values for MAb1 charge variants.

Peptides	Regions	Charge	Acidic peak (M GuHCl)	K0 (M GuHCl)	K1 (M GuHCl)	K2 (M GuHCl)
HC 53–65	HC Variable	1	N/A	N/A	N/A	N/A
HC 117–148	HC CH1	2	3.6	3.6	3.6	3.6
HC 150–178	HC CH1	3	3.7	3.6	3.6	3.6
			3.7*	3.6*	3.6*	3.6*
HC 246–256	HC CH2	1	N/A	N/A	N/A	N/A
HC 381–408	HC CH3	2	3.9	4.1	4.1	3.9
LC 1–32	LC Variable	3	3.6	3.7	3.7	3.6
LC 33–46	LC Variable	2	3.5	3.6	3.5	3.6
LC 47–70	LC Variable	2	3.5	3.5	3.6	3.5
LC 71–82	LC Variable	1	3.6	3.5	3.5	3.5
			3.6**	3.6**	3.6**	3.6**
LC 116–132	LC constant	2	3.5	3.6	3.6	3.6

^*Average C^1/2 _SUPREX value for HC CH1 region from HC 117–148 and 150–178. **Average C^1/2 _SUPREX value for LC variable region from LC 1–32, 33–46, 47–70 and 71–82. Experimental error for SURPEX measurement is typically ± 0.1 M GuHCl.