Figure 1. Schematic diagram showing the genetic constructs used in the production of trimerbody molecules. (A). All constructs bear a TIE domain composed of the N-terminal trimerization region of collagen XVIII NC1 (red box) flanked by one or two flexible linkers (yellow box). The trimerbody gene constructs contain a heterologous signal peptide from the oncostatin M (gray box), the scFv gene (VH and VL domains joined by a flexible linker) and a TIE^XVIII domain. Arrows indicate the direction of transcription. His6myc tag (purple box) appended was for immunodetection. (B). Schematic representation of the scFv-based N-, C- and N/C-terminal trimerbodies.