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. 2013 Jan 1;5(1):86–101. doi: 10.4161/mabs.22964

Table 3. Characterization data for FabRICATOR®-generated fragments.

FabRICATOR®-generated fragment monitored by SEC Approximate molar mass by SEC-MALLS
(g/mol)		 Observed MW by LC-MS
(Da)		 Assigned SEC Peak Identity
Peak 1 a
 200 x 103 g/mol
 98767 Da
(mass of Fabʹ2: Two disulfide-linked Fab arms)
 Fabʹ2/Fabʹ2 non-covalent dimer
Peak 2 b
 98 x 103 g/mol
 98769 Da
(mass of Fabʹ2: Two disulfide-linked Fab arms)
 Fabʹ2
Peak 3 c 50 x 103 g/mol 25232 Da
(mass of Fc fragment)		 Fc/Fc non-covalent dimer

a Peak 1 is only significantly present in the FabRICATOR® digest of the dimer sample. bPeaks 2 is very prominent in the FabRICATOR® digest of the monomer sample only; it is seen as a small minor peak in the Fabricator digest of the dimer. cPeaks 3 is observed equally in the FabRICATOR® digests of both the dimer and monomer sample. The Fc/Fc species is inherent to both samples, and is not indicative of the native dimer’s interface region.