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. 2013 Feb 5;8(2):e55848. doi: 10.1371/journal.pone.0055848

Figure 3. ASYN increases Tau phosphorylation in S396/404 via RIM11.

Figure 3

A) Relative percentage of phosphorylated tau (WT and P301L) in S396/404 labelled by the antibody AD2 was increased in the presence of ASYN (WT and A53T). (*p<0,05; **p<0,01). GAPDH was used as loading control. B) No phosphorylated tau (WT or P301L) detected in ΔRIM11 mutant by western blot when expressed alone or in combination with ASYN (WT or A53T). GAPDH was used as loading control. C) Lack of tau (WT and P301L) phosphorylation in S396/404 doesn’t alter cytotoxicity observed in solid media when tau (WT or P301L) and ASYN (WT and A53T) are expressed alone or in combination in ΔRIM11 as compared to expression in BY4741 WT.A quantitative plot of the dot spots is shown (*p<0,05; **p<0,01; ***p<0,001). Results are representative of at least three independent experiments.