Table 1.

Primary antibodies characteristics

Antibody/species	Supplier/code/lot	Immunogen	Dilution
Rabbit polyclonal anti-Notch Homolog 1, Translocation-associated (Notch1)	Lifespan Biosciences	Synthetic peptide CQHSYSSPVDNTPSHQ N-terminal added cysteine Human Notch1 intracell. domain (aa 2488–2502)	1:300
	LS-C16928
	Lot 6113061
Rabbit polyclonal anti-neurod (NeuroD1)	Lifespan Biosciences	Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH Human NeuroD1 (aa 76–91)	1:100
	LS-C9245
	Lot 8030526
Mouse monoclonal anti-Neuron specific beta III Tubulin [TUJ-1]	Abcam	Rat brain microtubules (whole Protein)	1:500
	ab14545
	Lot 543886
Rabbit polyclonal anti-Phospho-Histone H3	Upstate	Linear peptide corresponding to human Histone H3 at Ser10.	1:500
	06-570
Rabbit polyclonal anti-Green fluorescent protein	Invitrogen	GFP from the jellyfish Aequorea victoria	1:250
	A11122
Goath polyclonal anti- Patched	Santa Cruz	Peptide mapping at the N-terminus of patched of mouse origin.	1:50
	sc-6149
Mouse monoclonal anti-PAX7	Hybridoma bank	Recombinant chicken protein made E. coli (aa 352–523)	1:15
	PAX7
Rabbit polyclonal anti-sonic hedgehog	Santa Cruz	Rabbit polyclonal anti (aa 41–200) of shh of human origin.	1:50
	sc-9024
Mouse monoclonal anti-HNF3-ß	Hybridoma bank	Recombinant chicken protein made E. coli	1:20
	4C7