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Amide proton hydrogen exchange in HP7-H7F and CC9-H7F. Isotope-edited 1H-NMR spectra of 15N-labelled ferrous heme-bound HP7-H7F (top) and CC9-H7F (bottom) as a function of time after solvent exchange into 25 mM KaD2PO4 D2O buffer pD 6.5 at 20°C. Hydrogen exchange takes more than two hours to complete for the HP7-H7F complex while for CC9-H7F exchange is complete by the first time point in 30 minutes – a difference of at least a factor of ten in amide proton protection factor.
