Table 1. Rationale for chemicals used in genome-wide analysis.

Chemical	Mode of Action	Analogues in Chemotherapy
Methyl methanesulfonate	Alkylating agent; directly damages DNA bases	Nitrogen-mustard based (e.g., ifosfamide, chlorambucil); other (e.g., temozolomide)
Hydroxyurea	Inhibitor of ribonucleotide reductase; causes stalled replication forks due to reduced nucleotide pool	Hydroxyurea (i.e., marketed as Droxia, Hydrea)
Benomyl	Binds tubulin heterodimers preventing microtubule assembly	Vinblastine, vincristine, vinorelbine, vindesine, paclitaxel
Rapamycin	Binds FK506-binding protein to inhibit TORC1 signaling	Sirolimus and derivatives [e.g., everolimus (Afinitor); temsirolimus (Torisel)]