Figure 3 .

uORFs knockouts. Illustration of the manner in which uORFs were mutated. The upper track shows expression, the dotted line under exponential growth in YPAD, and the dashed line under salt stimulation conditions. The lower track shows the main ORF in solid black and the knocked-out uORFs in light gray. Out-of-register ORFs, which were not knocked out, are shown in dark gray. (A) ATP18; (B) GID7. In the case of GID7, the shorter of those two out-of-register ORFs is conserved across Saccharomyces paradoxus, Saccharomyces mikatae, and Saccharomyces bayanus (see sequence data from Cliften et al. 2003). Please also note that the exact insert placed into the plasmid ran from the far left of each panel to the beginning of the main ORF; the latter was replaced with luciferase.