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Luciferase analysis of uORFs. Luciferase assays confirming the relevance of uORFs in four genes with longer alternative 5′ UTRs in the salt stress condition. (A) ATP18; (B) HEM3; (C) GID7; and (D) TID3. The left two bars show results in SC–ura media and the right two bars show results in SC–ura media with 1 M salt for 1 hr. White bars are the results using the leader sequences, and gray bars have had uORF start codons mutated.
