Table 1. The 18 conditions under which RNA-Seq was performed.
| Condition | Description | No. of Sequenced Reads |
|---|---|---|
| Exponential growth | YPAD medium | 52,497,608 |
| Salt | 1 M NaCl for 45 min | 29,363,375 |
| DNA damage | 1 mM MMS for 1 hr | 29,427,647 |
| Alpha factor | 2.5 mM for 45 min; add another 50 μL to 25 mL yeast for another 30 min | 54,107,345 |
| Sorbitol | 1 M sorbitol for 45 min | 55,210,660 |
| Oxidative stress | 0.4 M H2O2 for 45 min | 34,262,447 |
| Heat shock | in 37° shaker for 1 hr | 36,362,297 |
| Stationary phase | 18 d in 30° incubator | 32,982,185 |
| SC media | Synthetic complete medium | 30,783,099 |
| SC glycerol media | 4% glycerol instead of glucose in SC medium | 35,241,114 |
| High calcium | 10 mM calcium chloride medium | 37,389,705 |
| Low nitrogen | 1/5 the normal amount of Yeast Nitrogen Base in YPAD | 27,864,332 |
| Low phosphate | see File S1 | 30,373,844 |
| Calcofluor | 0.1% for 1 hr | 31,921,767 |
| Hydroxyurea | 0.075M for 1 hr | 27,825,606 |
| Grape juice | Walgreen’s brand grape juice, filtered | 50,208,736 |
| Benomyl | 5 μg/mL for 1 hr | 40,974,093 |
| Congo red | 30 μg/mL of Congo red for 1 hr | 33,650,224 |
Unless otherwise noted, the conditions consist of YPAD medium with the indicated reagents added for the indicated incubation time. SC, synthetic complete.