Table 1. Sequencing effort and assembly information for C. solstitialis normalized Roche 454 transcriptome libraries.

						Raw	Cleaned			Assembled
SampleTissue [Latitude, Longitude]	Platform	Plates	Mb	Mb	Read No.	Median bp	Mb	Unigene No.	Median bp	Contig No.	UCO No.
North America (introduced)
CA-1-1S [N 41° 59’, W 122° 36’]	FLX	0.25	17.2	13.0	69939	211	2.4	9783	242	8769	10
CA-1-2S [N 41° 59’, W 122° 36’]	FLX	0.25	25.1	19.0	101468	213	3.2	12810	242	11237	18
CA-2-2S [N 40° 25’, W 122° 16’]	FLX	0.25	20.1	15.9	82739	216	2.8	10596	255	9236	25
CA-2-4S [N 40° 25’, W 122° 16’]	FLX	0.25	19.9	14.3	78770	210	2.4	9853	238	8551	14
CA-3-1S [N 39° 12’, W 121° 06’]	FLX	0.5	58.0	45.6	229635	224	7.1	23671	268	19905	77
CA-3-2S [N 39° 12’, W 121° 06’]	FLX	0.5	58.1	39.9	212773	219	5.8	21578	254	18377	28
CA-4-1S [N 38° 31’, W 121° 45’]	FLX	0.5	80.1	66.8	321955	226	10.1	32206	270	27121	109
CA-4-3S [N 38° 31’, W 121° 45’]	FLX	0.25	20.0	13.2	72154	214	2.3	9524	238	8655	11
CA-4-4S [N 38° 31’, W 121° 45’]	Ti	0.75	206.3	193.2	579859	366	24.8	42042	537	32786	252
CA-5-3S [N 38° 16’, W 121° 49’]	FLX	0.5	80.2	65.5	325569	223	9.9	32743	266	27222	111
CA-5-4S [N 38° 16’, W 121° 49’]	FLX	0.25	21.1	14.7	79481	215	2.3	9172	249	8112	13
South America (introduced)
AR-1-24L [S 36° 26’, W 64° 17’]	Ti	0.5	234.7	225.4	542881	462	33.4	46538	632	38346	299
AR-1-25CL [S 36° 26’, W 64° 17’]	Ti	0.56	153.7	132	369437	412	21.1	39579	532	29944	177
AR-6-13L [S 37° 39’, W 64° 08’]	Ti	0.5	256.4	247.1	596060	473	28.5	38572	630	31082	287
AR-6-26L [S 37° 39’, W 64° 08’]	Ti	0.5	181.7	169.2	485202	390	17.3	30641	537	23376	185
AR-8-15L [S 38° 11’, W 64° 04’]	Ti	0.5	166.1	154.6	443044	391	20.4	35628	549	26419	230
AR-8-19L [S 38° 11’, W 64° 04’]	Ti	0.5	259.3	249.9	600878	458	28.7	37199	673	30305	303
AR-13-24L [S 36° 18’, W 65° 40’]	Ti	0.5	274.6	264.7	622982	491	28.4	37408	679	29348	311
AR-13-28L [S 36° 18’, W 65° 40’]	Ti	0.63	183.5	171.5	488746	405	21.1	38160	540	28579	206
Western Europe (putative ancient expansion)
SP-1-5L [N 39° 50’, W 2° 30’]	Ti	0.5	152.5	135.6	393803	398	19.5	38699	519	27841	172
SP-1-10L [N 39° 50’, W 2° 30’]	Ti	0.5	269.7	258.7	604697	483	29.5	43236	637	32884	292
SP-2-2L [N 41° 45’, W 4° 5′]	Ti	0.63	181.9	162.1	522160	341	22.8	46071	474	34707	184
SP-2-6L [N 41° 45’, W 4° 5′]	Ti	1	332.6	315.9	815538	459	41.6	62444	583	48230	311
Eastern Europe (native)
GA-1-1S [N 41° 56’, E 45° 27’]	FLX	0.25	19.1	12.5	71584	209	2.0	8901	233	7823	8
GA-2-1S [N 41° 56’, E 45° 35’]	FLX	0.25	18.9	12.9	73656	210	2.0	8921	233	7659	11
GA-3-4S [N 41° 44’, E 45° 12’]	FLX	0.25	11.5	7.5	42595	212	1.3	5257	240	4704	3
GA-4-3S [N 41° 43’, E 45° 16’]	FLX	0.25	13.0	7.7	43567	215	1.3	5299	240	4811	11
GA-5-4S [N 41° 38’, E 45° 38’]	FLX	0.25	12.8	8.6	48417	214	1.5	6073	243	5407	4
GA-5-24L [N 41° 38’, E 45° 38’]	Ti	0.63	153.5	126.5	367110	390	17.4	33086	522	25382	165
HU-1-8L [N 46° 58’, E 18° 41’]	Ti	0.5	185.3	172.4	465081	421	22.3	39661	559	28967	213
HU-2-10L [N 47° 19’, E 21° 02’]	Ti	0.5	297.9	286.6	661490	489	32.5	40448	699	32643	311
RO-1-6L [N 47° 42’, E 26° 2’]	Ti	0.75	189.6	170.8	518480	376	22.6	43834	510	32820	182
RO-5-10L [N 46° 15’, E 27° 39’]	Ti	0.5	292.5	279.1	663787	479	33.7	52108	602	39456	293
TK-1-3S [N 39° 45’, E 29° 06’]	FLX	0.5	24.7	17.3	89230	229	3.2	11053	268	9841	26
TK-1-5L [N 39° 45’, E 29° 06’]	Ti	0.63	159.5	145.6	494855	327	20.2	41229	462	30361	207
TK-2-3S [N 37° 02’, E 29° 47’]	FLX	0.25	17.1	10.6	63668	201	1.8	8085	223	7106	4
TK-2-4S [N 37° 02’, E 29° 47’]	FLX	0.25	15.7	10.0	59734	204	1.5	7087	225	6191	6
TK-3-2S [N 37° 50’, E 27° 51’]	FLX	0.25	11.4	6.2	38205	198	1.0	4873	210	4373	2
TK-5-4S [N 37° 01’, E 30° 22’]	Ti/FLX	2.25	430.7	389.4	1258319	352	35.1	71045	418	48608	278
TK-5-9L [N 37° 01’, E 30° 22’]	Ti	0.5	252.1	241.7	554227	481	29.8	40147	671	32773	283
Pseudo-reference							39.6	43717	811		260
Pseudo-reference filtered for polymorphic single loci							21.2	22687	840		154

Tissues included are whole seedlings (S) or leaves (L). FLX, 454 Life Science Genome Sequencer FLX; Ti, FLX-Titanium.