Table 3.
Effects of protopine and allocryptopine on TAM metabolism
| % Inhibition | |||
|---|---|---|---|
| Compound | 4-OH TAM | N-desmethyl TAM | α-OH TAM |
| Protopine (10 µM) | 63.4±7.7a | 15.8±1.3 | 12.4±6.3 |
| Allocryptopine (10 µM) | 57.9±4.3 | 16.8±2.8 | < 10 |
| Protopine+Allocryptopineb | 32.1±6.3 | < 10 | < 10 |
| FCPC Fraction 6 (50 µg/ml) | 62.1±3.0 | 76.5±3.0 | 72.8±1.1 |
| Quinidine (1 µM) | 57.6±1.9 | < 10 | < 10 |
a
Data represent average of triplicates ±S.D.
b
Alkaloids were mixed in concentrations corresponding to those in FCPC Fraction 6. At 50 µg/ml of Fraction 6, the concentrations of protopine and allocryptopine were 53 and 61 nM, respectively.