Figure 2.
MCP-1 production by HUVEC following stimulation with IgG from subjects with elevated (IgG aCl >15 U/ml) or normal ((IgG aCl >15 U/ml) aCl titers. 20 ug of IgG were added from each sample per well and incubated at 37°C in 5% CO2 for 24h. Experiments were carried out in triplicate and the mean concentration of MCP-1 in culture supernatants was determined. MCP-1 produced by HUVEC was significantly greater for IgG from sera containing elevated aCl titers than for those with normal aCl titers (420.7 ug/ml versus 152.1 ug/ml, p<.0001). Diamonds illustrate mean values and confidence intervals.