Fig. 1.

Chromosomal association of newly expressed HA-MeCP2. Newly made HA-MeCP2 in stage IV–V Xenopus oocytes targets the chromomeric regions of LBCs. DAPI staining (in red) shows that the chromosomal association of MeCP2 is proportional to the DNA density. While many lateral loops are present (they are readily visible by DIC), they appear to be devoid of HA-MeCP2. However, very short HA-MeCP2 positive lateral projections are readily visible and are likely to correspond to the bases of many loops. HA-MeCP2 also associates with nucleoli, but at a low concentration and primarily with the granular region