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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Cells Tissues Organs. 2004;176(1-3):95–108. doi: 10.1159/000075031

Fig. 7.

Fig. 7

Cotransduction of mesenchymal cell lines with adenoviruses expressing combinations of BMPs synergistically stimulates alkaline phosphatase activity. C3H10T1/2 and ST2 cells were transduced with the indicated adenoviruses. Cells were harvested after 8 days for measurement of alkaline phosphatase activity and DNA (for normalization). Cotransduction of cells with combinations of either AdCMV-BMP2 or AdCMV-BMP4 with AdCMV-BMP7 stimulated alkaline phosphatase 2–4 times more than would be predicted if effects of individual adenoviruses were additive. 10T1/2 = C3H10T1/2 mouse mesenchymal cell line; LacZ = AdCMV-lacZ; B2 = AdCMV-BMP2; B4 = AdCMV-BMP4; B7 = AdCMV-BMP7. Significantly different from the sum of activity in cells individually transduced with AdCMV-BMP2 and AdCMV-BMP7, * p < 0.001; significantly different from the sum of activity in cells individually transduced with AdCMV-BMP4 and AdCMV-BMP7, + p < 0.001.