FIGURE 2.

Protein secretion profile correlates to the stage of cancer stem cell differentiation. (A) Heat map showing the protein secretion profiles measured at different times during the differentiation of human glioma stem cells in vitro. (B) Schematic depiction of the stage of glioma stem cell differentiation as identified by emergence of cytokine secretion. In our protein assay panel, glioma stem cells appear to be relatively “quiescent.” During the differentiation process, these cells begin to produce a number of functional proteins and gradually become the phenotype of mature tumor cells.