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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Biochemistry. 2010 Nov 22;49(50):10728–10736. doi: 10.1021/bi1015716

Figure 3.

Figure 3

EMSA of pri-miR-16-1 binding to DGCR8-dsRBD1. (A) Predicted secondary structure of pri-miR-16-1 with the sequence of the mature miRNA shown in red and the region removed by Drosha cleavage indicated through lower case letters. (B) Representative gel showing addition of DGCR8-dsRBD1 (2–200 µM) to 0.25 nM pri-miR-16-1. (C) Fitted EMSA fraction bound as a function of DGCR8-dsRBD1 concentration with data points and uncertainties represented by filled circles and the best fit to the data (see text) represented as a gray line.