Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Dec 21;14(1):255–272. doi: 10.3390/ijms14010255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Decreased CrAT activity disrupts mitochondrial bioenergetics and ATP generation in pulmonary arterial endothelial cells. PAEC were transiently transfected with a scrambled siRNA or a specific CrAT siRNA for 48 h and the effect on mitochondrial respiration determined using the Seahorse XF24 analyzer. In the CrAT siRNA transfected cells there was a significant decrease in the (A) amount of oxygen consumed (OCR) for ATP production and (B) this corresponded with a reduction in cellular ATP levels. Values are mean ± SEM; N = 6. *p < 0.05 vs. scrambled siRNA.