Figure 4.

CrAT gene silencing causes nitrative stress in pulmonary arterial endothelial cells. PAEC were transiently transfected with a scrambled siRNA or a specific CrAT siRNA for 48 h and the effect on cellular peroxynitrite levels determined. There was an increase in peroxynitrite generation (A) as determined by DHR123 oxidation and a corresponding increase in total nitrated proteins; (B) as determined by Dot Blot analysis (C) This increase in peroxynitrite generation resulted in a significant increase of SOD2 nitration. Values are mean ± SEM; N = 6–11. *p < 0.05 vs. scrambled siRNA.