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MDC-labeled autophagic vacuoles in PC-2 cells by fluorescence microscope after treatment with Rapa. Autophagic vacuoles were labeled with 0.05 mmol/L MDC in phosphatebuffered saline (PBS) at 37 °C for 10 min. (A) 0 nmol/L Rapa group; (B) 10 nmol/L Rapa group; (C) 50 nmol/L Rapa group; (D) 50 nmol/L Rapa + 3-MA group (200×).
