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. 2012 Dec 21;14(1):273–285. doi: 10.3390/ijms14010273

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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The mRNA expression of mTOR, p53, Bax and Beclin 1 in PC-2 cells treated with different concentrations of Rapa: After treatment with different doses of Rapa for 24 h, mRNA level was detected by semi-quantitive RT-PCR analysis. This assay was done quintuplicate. Values represent means ± standard deviations and were determined using the Student’s t-test. * p < 0.05 and ** p < 0.01 versus 0 nmol/L Rapa group. (a) 0 nmol/L Rapa group; (b) 10 nmol/L Rapa group; (c) 30 nmol/L Rapa group; (d) 50 nmol/L Rapa group.