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. 2012 Dec 28;14(1):611–624. doi: 10.3390/ijms14010611

Figure 2.

Figure 2

The disrupted locus in mutant B7 encodes a developmentally regulated transcript whose expression in significantly reduced in mutant parasites. (A) Schematic representation of T. gondii chromosome VI, positions 65,000 to 71,000. The location of the insertion in mutant B7 and mutant 29C3 (described below in the text) is shown. The genes/transcripts present in this region of chromosome VI are also shown; (B) The expression of Tg-ncRNA-1 was examined by Real Time PCR. Total RNA was extracted from wild-type (WT) and mutant (B7) under tachyzoite growth conditions (Tachy), 2 days and 4 days after bradyzoite induction (Brady-2 and Brady-4 respectively). The values indicate the relative gene expression levels normalized to the expression levels of α-tubulin (constitutively expressed gene-endogenous control). The significance of the data was determined by Student’s t-test (* p < 0.05, ** p < 0.01 and *** p < 0.001). The data are represented as mean ± SEM. The expression of TGME49_038100 and TGME49_038110 is not affected in the mutant parasites or developmentally regulated.