Figure 3.

Complementation of mutants B7 and 29C3 rescues the wild-type phenotype. Confluent HFF cells were infected with the wild-type (WT), mutant (B7 or 29C3), and complemented parasite lines (B7.Compl.1 or B7.Compl.2 or 29C3.Compl); and subjected to bradyzoite differentiation conditions for 72 h. (A) and (C) The presence/absence of bradyzoite marker expression (BAG1 and DL) was counted for 100 parasite vacuoles, in three independent experiments. (B) and (D) Proliferation was determined by counting the number of parasites per vacuole (in 100 vacuoles), in three independent experiments. The significance of the data was determined by Student’s t-test (* p < 0.05, ** p < 0.01 and *** p < 0.001). The data are represented as mean ± SEM.