Figure 5.

Another independent insertional mutant (29C3) containing an insertion in Tg-ncRNA-1 is defective in bradyzoite differentiation. Confluent HFF cells were infected with the wild-type (WT) and mutant parasites (29C3) and subjected to bradyzoite differentiation conditions for 72 h. (A) The presence/absence of bradyzoite marker expression (BAG1 and DL) was counted for 100 parasite vacuoles, in three independent experiments; (B) Proliferation was determined by counting the number of parasites per vacuole (in 100 vacuoles), in three independent experiments. The location of the insertion in 29C3 is shown in Figure 2A. The significance of the data was determined by Student’s t-test (* p < 0.05, ** p < 0.01 and *** p < 0.001). The data are represented as mean ± SEM.