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. 2013 Jan 7;14(1):935–953. doi: 10.3390/ijms14010935

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effects of OTA on blastocyst viability. Mouse blastocysts were treated with OTA (1, 5, or 10 μM) for 24 h, or left untreated. (A) The total number of cells per blastocyst, and the numbers of cells in the inner cell mass (ICM) and the trophectoderm (TE) were counted; (B) The proportions of Annexin V-positive/PI-negative cells in the blastocysts of each group were determined. Data are based on at least 230 blastocysts from each group. Values are presented as means ± SEMs of five separate experiments. ***p < 0.001 versus the control group.